Lets assume you follow the protocol exactly and take meticulous care to not contaminate. These are available online in convenient and compact pdf format at. With the rneasy mini kit, total rna is easily purified from 10 to 1 x 10 7 animal or human cells, 0. Total rna isolation with rneasy micro qiagen spin protocol.
Rneasy mini kit for purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for rna cleanup. The rneasy plus mini kit should be stored dry at room temperature 1525. Rnasefree water directly to the spin column membrane. No claim or representation is intended for their use to identify any specific organism or for a specific clinical use diagnostic, prognostic, therapeutic, or blood banking. Animal cells using the rneasy plus mini kit and rneasy minelute cleanup kit. Rneasy mini protocol for isolation of total rna from heart, muscle. For phenolfree total rna extraction from cells tissues with removal of genomic dna contamination. Reagent by using the rneasy plus mini kit along with a protocol which is available. Rna extraction from plants using the rneasy plant mini kit. Quickstartprotocol rneasy plus mini kit january 2011 igem 2016. Drop 2 x 2 l rnase free water as blank on the first row, and 2 x 2 l rna on the.
Rneasy mini handbook 042006 7 the rneasy mini kit and rneasy protect mini kit are intended as generalpurpose devices. These are available online in convenient and compact pdf. The rneasy plus mini kit, which is designed for rna purification from animal cells and. C and is stable for at least 9 months under these conditions. Rneasy plant mini kit is ideal for isolating total rna from complex, fiberrich and difficulttohomogenize samples now you can tackle tough tissue types. Rneasy protect mini kit for immediate stabilization of rna in harvested animal tissues and subsequent total rna purification. Rneasy minihandbook rneasy mini kit for purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for rna cleanup. Optional oncolumn dnase digestion with the rnasefree dnase. We reserve the right to change, alter, or modify any product to enhance its performance. Rneasy plant mini kit for purification of total rna from plants and filamentous fungi. Quickstartprotocol rneasy mini kit, part 1 january 2011. Rna isolation protocol using the qiagen rneasy mini kit materials and preparation of solutions.
Rnase free water 10 ml 50 ml quickstart protocol 11 rneasyprotectminikit 50 250. Product use limitations the rneasy plus mini kit is intended for molecular biology applications. Rneasy plus mini kit 250 from qiagen sample to insight. For technical assistance, please call tollfree 0080022446000, or find. Tips for rna extraction with rneasy kit from qiagen.
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